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Systemic and CSF Interleukin-1alpha expression in a rabbit closed cranium subarachnoid haemorrhage model
EANS Academy. Croci D. Sep 27, 2019; 275951; EP01075
Abstract
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Background and aim: Delayed cerebral vasospasm (DCVS) and delayed cerebral ischemia (DCI) are severe complication of subarachnoid haemorrhage (SAH). Inflammation due to blood haemolysis in cerebrospinal fluid (CSF) has been recognized to be one of the leading factor causing DCVS and DCI. IL-1ahas been reported to be important in the initiation and maintenance of inflammation in different human diseases and the initiation of the sterile inflammatory response. IL-1aand bare considered major key players in initiating the inflammation cascade after stroke. Notably, IL-1ahas been described to be early expressed in areas of focal neuronal injury after stroke and precede the expression of IL-1band IL-6. The detailed role of IL-1aafter SAH, however, has not been evaluated. The aim of our study is to analyse systemic and CSF changes of IL-1ain a rabbit closed cranial subarachnoid haemorrhage model.
Methods: 17 New Zealand white rabbits were randomized in two groups: 1) SAH (n=12); 2) sham (n=5). In the first group SAH was induced by extracranial-intracranial shunting from the subclavian artery into the cerebral cistern of magna under intracranial pressure (ICP) monitoring. The second group was used as control without SAH induction. CSF and blood samples were taken at day 0 before SAH induction and at day 3. IL-1alevels in plasma and CSF were measured with specific ELISA-Kit.
Results: There was a significant increase of ICP (p=0.00009) and decrease of CPP (p= 0.00089)during SAH induction. At follow up there was a significant higher level of systemicIL-1ain the SAH as compared to the Sham group (p=0.042). There was no statistical significant difference in CSF values in both group.
Conclusions: Systemic IL-1alevels are elevated after SAH induction in a rabbit SAH model. Further studies are warranted to evaluate the effect of IL-1aon the occurrence of DCVS and DCI after SAH.
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